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Image Search Results
Journal: Human Molecular Genetics
Article Title: XIAP and cIAP1 amplifications induce Beclin 1-dependent autophagy through NFκB activation
doi: 10.1093/hmg/ddv052
Figure Lengend Snippet: High levels of XIAP in B-cell lymphoma activate autophagy. ( A ) B cells (WT) and three diffuse large B-cell lymphoma cell lines, SUDHL5, SUDHL8 and SUDHL10 were subjected to immunoblotting with XIAP and actin antibodies. ( B ) The same cell lines were treated with 400 n m bafilomycin A1 and were subsequently subjected to immunoblotting with LC3 and tubulin antibodies. The diffuse large B-cell lymphoma cell lines, SUDHL5 ( C ), SUDHL8 ( D ) and SUDHL10 ( E ), were treated without or with 10 μM embelin (Emb) for 16 h, and treated without or with 400 n m bafilomycin A1 (Baf A1) for the last 4 h of the experiment, and were subsequently subjected to immunoblotting with LC3 and tubulin antibodies. ( F ) Propidium iodide and FITC-conjugated Annexin A5 staining detected by flow cytometry of B cells (WT) SUDHL5, SUDHL8 and SUDHL10 (5, 8 and 10) treated without or with 10 μM embelin (Emb) for 16 h. The percentage of cells positive for both PI and Annexin A5 are shown. Densitometric measurements of LC3-II bands were normalized to the corresponding actin or tubulin bands and are shown in the corresponding histograms. The values shown in all the histograms represent the mean ± standard deviation from at least three independent experiments performed in triplicate samples/condition. The P -values were determined using one sample t -tests, where controls are set to 100%. See also Supplementary Material, Figure S7 .
Article Snippet: Wild-type B-cells and
Techniques: Western Blot, Staining, Flow Cytometry, Standard Deviation